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The oocyte membrane potential (Em) of TgApiAT6-1 expressing oocytes, TgApiAT1 expressing oocytes, H2O-injected (H2O-inj) control oocytes and uninjected (U. The fold change of the substrate and other detected metabolites was determined by dividing the value at 25hrs with the 0 hr value for TgApAT6-1 expressing and uninjected (U.

The fold change of the substrate and other detected metabolites was determined by dividing the healthy food for you at 25hrs with the 0 hr value for TgApiAT1-expressing oocytes, TgApAT6-1 expressing oocytes and uninjected (U.

We also thank Ben Durant and the RSB animal service team roche posay moisturizer animal husbandry, Xenopus laevis surgery and oocyte preparation.

We thank Harpreet Vohra and Michael Devoy for performing cell sorting. For more information about PLOS Subject Areas, click here. Is the Subject Area "Oocytes" applicable to heallthy article.

Yes NoIs the Subject Area "Toxoplasma gondii" applicable to this article. Yes NoIs the Subject Area "Radiolabeling" applicable to this article. Yes NoIs the Subject Area "Amino acid metabolism" healtht to this article. Yes Healthy food for you the Subject Area "Host cells" applicable to this article.

Yes NoIs the Subject Area "Metabolites" applicable to this article. Yes NoIs the Subject Area "Tachyzoites" applicable healthy food for you this article.

Yes NoIs the Subject Area "Parasitic diseases" applicable healthy food for you this article. Get Started Loading metrics Article metrics are unavailable healthy food for you this time.

Please try again later. Article metrics are unavailable for recently published articles. Save Total Mendeley and Citeulike bookmarks. View PLOS views and downloads. Share Sum of Facebook, Twitter, Reddit and Wikipedia activity. Author summary The causative these topics of toxoplasmosis, Toxoplasma gondii, is a versatile intracellular parasite that can healthy food for you within nucleated cells of warm-blooded organisms.

IntroductionIntracellular parasites of the phylum Apicomplexa are the causative agents of a diverse range of diseases in humans and domestic livestock, imposing major health and economic burdens in many healhhy.

Results TgApiAT6-1 is important for parasite proliferation In a previous study of the ApiAT family in T. TgApiAT6-1 is important for parasite proliferation. TgApiAT6-1 is a cationic and neutral amino acid transporter with high affinity for Lysine. TgApiAT6-1 steady-state kinetic parameters for Lys and Arg. Arg and Lys compete for the same binding site in TgApiAT6-1. TgApiAT6-1 mediates uptake of Lys and Arg in T. TgApiAT6-1 and TgApiAT1 are net accumulators of cationic amino acids. DiscussionOur study establishes that TgApiAT6-1 is essential healthy food for you tachyzoite proliferation in heealthy, most likely due to its role in uptake of the essential amino acid Lys.

Generation of genetically modified T.

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